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5 Simple Statements About DNA methylation test Explained

treatment method with sodium bisulfite deaminates unmethylated cytosine to uracil, While 5mC isn't impacted. Subsequent PCR amplification will change the uracils by thymines. Reads from NGS libraries developed in parallel with and without bisulfite therapy are mapped onto reference genomes as well as contrasting C/T positions involving the librarie

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